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Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.

Donnelly, Daniel P; Rawlins, Catherine M; DeHart, Caroline J; Fornelli, Luca; Schachner, Luis F; Lin, Ziqing; Lippens, Jennifer L; Aluri, Krishna C; Sarin, Richa; Chen, Bifan; Lantz, Carter; Jung, Wonhyeuk; Johnson, Kendall R; Koller, Antonius; Wolff, Jeremy J; Campuzano, Iain D G; Auclair, Jared R; Ivanov, Alexander R; Whitelegge, Julian P; Pasa-Tolic, Ljiljana; Chamot-Rooke, Julia; Danis, Paul O; Smith, Lloyd M; Tsybin, Yury O; Loo, Joseph A; Ge, Ying; Kelleher, Neil L; Agar, Jeffrey N.
Nat Methods; 16(7): 587-594, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31249407
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
Selo DaSilva