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Next-generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut (Arachis hypogaea L.).

Luo, Huaiyong; Pandey, Manish K; Khan, Aamir W; Wu, Bei; Guo, Jianbin; Ren, Xiaoping; Zhou, Xiaojing; Chen, Yuning; Chen, Weigang; Huang, Li; Liu, Nian; Lei, Yong; Liao, Boshou; Varshney, Rajeev K; Jiang, Huifang.
Plant Biotechnol J; 17(12): 2356-2369, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31087470
Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic markers. Here, we deployed sequencing-based trait mapping approach, QTL-seq, to discover genomic regions, candidate genes and diagnostic markers for BWR in a recombination inbred line population (195 progenies) of peanut. The QTL-seq analysis identified one candidate genomic region on chromosome B02 significantly associated with BWR. Mapping of newly developed single nucleotide polymorphism (SNP) markers narrowed down the region to 2.07 Mb and confirmed its major effects and stable expressions across three environments. This candidate genomic region had 49 nonsynonymous SNPs affecting 19 putative candidate genes including seven putative resistance genes (R-genes). Two diagnostic markers were successfully validated in diverse breeding lines and cultivars and could be deployed in genomics-assisted breeding of varieties with enhanced BWR.
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