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Draft Genome Sequence Resource of Switchgrass Rust Pathogen, Puccinia novopanici Isolate Ard-01.

Gill, Upinder S; Nandety, Raja Sekhar; Krom, Nick; Dai, Xinbin; Zhuang, Zhaohong; Tang, Yuhong; Zhao, Patrick X; Mysore, Kirankumar S.
Phytopathology; 109(9): 1513-1515, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31050598
Puccinia novopanici is an important biotrophic fungal pathogen that causes rust disease in switchgrass. Lack of genomic resources for P. novopanici has hampered the progress toward developing effective disease resistance against this pathogen. Therefore, we have sequenced the whole genome of P. novopanici and generated a framework to understand pathogenicity mechanisms and identify effectors, repeat element invasion, genome evolution, and comparative genomics among Puccinia spp. in the future. Long- and short-read sequences were generated from P. novopanici genomic DNA by PacBio and Illumina technologies, respectively, and assembled a 99.9-Mb genome. Transcripts of P. novopanici were predicted from assembled genome using MAKER and were further validated by RNAseq data. The genome sequence information of P. novopanici will be a valuable resource for researchers working on monocot rusts and plant disease resistance in general.
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