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Diabetes relief in mice by glucose-sensing insulin-secreting human α-cells.

Furuyama, Kenichiro; Chera, Simona; van Gurp, Léon; Oropeza, Daniel; Ghila, Luiza; Damond, Nicolas; Vethe, Heidrun; Paulo, Joao A; Joosten, Antoinette M; Berney, Thierry; Bosco, Domenico; Dorrell, Craig; Grompe, Markus; Ræder, Helge; Roep, Bart O; Thorel, Fabrizio; Herrera, Pedro L.
Nature; 567(7746): 43-48, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30760930
Cell-identity switches, in which terminally differentiated cells are converted into different cell types when stressed, represent a widespread regenerative strategy in animals, yet they are poorly documented in mammals. In mice, some glucagon-producing pancreatic α-cells and somatostatin-producing δ-cells become insulin-expressing cells after the ablation of insulin-secreting ß-cells, thus promoting diabetes recovery. Whether human islets also display this plasticity, especially in diabetic conditions, remains unknown. Here we show that islet non-ß-cells, namely α-cells and pancreatic polypeptide (PPY)-producing γ-cells, obtained from deceased non-diabetic or diabetic human donors, can be lineage-traced and reprogrammed by the transcription factors PDX1 and MAFA to produce and secrete insulin in response to glucose. When transplanted into diabetic mice, converted human α-cells reverse diabetes and continue to produce insulin even after six months. Notably, insulin-producing α-cells maintain expression of α-cell markers, as seen by deep transcriptomic and proteomic characterization. These observations provide conceptual evidence and a molecular framework for a mechanistic understanding of in situ cell plasticity as a treatment for diabetes and other degenerative diseases.
Selo DaSilva