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Response of Paracoccidioides lutzii to the antifungal camphene thiosemicarbazide determined by proteomic analysis.

E Silva, Kleber Sf; da S Neto, Benedito R; Zambuzzi-Carvalho, Patrícia F; de Oliveira, Cecília Ma; Pires, Ludmila B; Kato, Lucilia; Bailão, Alexandre M; Parente-Rocha, Juliana A; Hernández, Orville; Ochoa, Juan Gm; de A Soares, Célia M; Pereira, Maristela.
Future Microbiol; 13: 1473-1496, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30311782

AIM:

To perform the proteomic profile of Paracoccidioides lutzii after treatment with the compound camphene thiosemicarbazide (TSC-C) in order to study its mode of action.

METHODS:

Proteomic analysis was carried out after cells were incubated with TSC-C in a subinhibitory concentration. Validation of the proteomic results comprised the azocasein assay, western blot and determination of the susceptibility of a mutant to the compound.

RESULTS:

Proteins related to metabolism, energy and protein fate were regulated after treatment. In addition, TSC-C reduces the proteolytic activity of the protein extract similarly to different types of protease inhibitors.

CONCLUSION:

TSC-C showed encouraging antifungal activity, working as a protease inhibitor and downregulating important pathways impairing the ability of the fungi cells to produce important precursors.
Selo DaSilva