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Preparation of Cross-Linked Chromatin for ChIP.

Kim, Tae Hoon; Dekker, Job.
Cold Spring Harb Protoc; 2018(4)2018 04 02.
Artigo em Inglês | MEDLINE | ID: mdl-29610358
After living cells have been treated with formaldehyde to lock proteins to their DNA substrates, the DNA-protein complexes must be extracted and fragmented as described here. For ChIP-chip, it is not essential that a majority of chromatin fragments are ∼500 bp; an average fragment size ranging 1-2 kb is sufficient. However, for ChIP-seq, the average chromatin fragment should be <500 bp.
Selo DaSilva