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Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay.

Liu, Liyuan; Jiang, Luzhi; Yu, Yongxin; Xia, Xiaoming; Pan, Yingjie; Yan, Shuling; Wang, Yongjie.
Mol Cell Probes; 33: 4-7, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28179103
A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.
Selo DaSilva