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Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing.

Liu, Xian Shuang; Fan, Bao Yan; Pan, Wan Long; Li, Chao; Levin, Albert M; Wang, Xinli; Zhang, Rui Lan; Zervos, Thomas M; Hu, Jiani; Zhang, Xiao Ming; Chopp, Michael; Zhang, Zheng Gang.
RNA Biol; 14(5): 488-499, 2017 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-27315491
Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions.
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