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CRISPR-Mediated Base Editing without DNA Double-Strand Breaks.

Plosky, Brian S.
Mol Cell; 62(4): 477-8, 2016 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-27203175
Targeting point mutations using CRISPR/Cas9 so far has required efficient homologous recombination (HR) and donor oligonucleotides. In a recent Nature paper, Komor and colleagues (2016) describe a way to make specific base changes that does not depend on HR or donor DNA and does not involve making double-strand breaks.
Selo DaSilva