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In vitro CRISPR-Cas9-mediated efficient Ad5 vector modification.

Tang, Lichun; Gong, Mengmeng; Zhang, Pumin.
Biochem Biophys Res Commun; 474(2): 395-399, 2016 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-27125457
The CRISPR-Cas9 genome editing system has been widely used in multiple cells and organisms. Here we developed a CRISPR-Cas9 based in vitro large DNA vector editing system, using the Ad5-based vector as an example. We demonstrate use of this system to generate targeted mutations, in-frame gene deletion, and gene replacement. This in vitro CRISPR editing system exhibits high efficiency and accuracy. We believe this system can be applied in a variety of experimental settings.
Selo DaSilva