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Quantitative temporal viromics: an approach to investigate host-pathogen interaction.

Weekes, Michael P; Tomasec, Peter; Huttlin, Edward L; Fielding, Ceri A; Nusinow, David; Stanton, Richard J; Wang, Eddie C Y; Aicheler, Rebecca; Murrell, Isa; Wilkinson, Gavin W G; Lehner, Paul J; Gygi, Steven P.
Cell; 157(6): 1460-72, 2014 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-24906157
A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called "quantitative temporal viromics" (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellular proteins, including 1,200 cell-surface proteins to manipulate signaling pathways and counterintrinsic, innate, and adaptive immune defenses. QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets. Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined. QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.
Selo DaSilva