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A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia.

Gallo, Stephanie Wagner; Ramos, Patrícia Locosque; Ferreira, Carlos Alexandre Sanchez; Oliveira, Sílvia Dias de.
Mem Inst Oswaldo Cruz; 108(3)2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23778655
Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
Selo DaSilva