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[Establishment of an internal control for directed differentiation using pluripotent stem cell lines derived from heterozygotic twins].

Luo, Yu-mei; Fan, Yong; Chen, Xin-jie; Yue, Lei; Li, Qing; He, Wen-zhi; Ma, Xiao-yan; Zheng, Yu-hong; Sun, Xiao-fang.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 29(4): 398-403, 2012 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-22875494

OBJECTIVE:

To reprogram amniotic fluid cells into pluripotent stem cells in order to create an optimal internal control model for directed cell differentiation.

METHODS:

Human amniotic fluid-derived cells (hAFDCs) from heterozygotic twin fetuses were induced by retroviral vectors encoding Oct4, Sox2, c-Myc and Klf4. In vivo pluripotency, differentiation capacity and karyotype of hAFDCs induced pluripotent stem cells (hAFDCs-iPSCs) were determined.

RESULTS:

hAFDC-iPSCs derived from heterozygotic twins have maintained self renewal, with expression of high pluripotency marker gene detected at both mRNA and protein levels. The cells have maintained their differentiation capacity both in vitro and vivo, and showed normal karyotypes after long-term culturing in vitro.

CONCLUSION:

hAFDCs-iPSCs derived from heterozygotic twins have good consistency in terms of genetic background, and can provide a good internal control for directed differentiation of iPSCs, and may be used an ideal source for autologous cell replacement therapy in the later life of the fetus.
Selo DaSilva