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Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma.

Agnelli, Luca; Mereu, Elisabetta; Pellegrino, Elisa; Limongi, Tania; Kwee, Ivo; Bergaggio, Elisa; Ponzoni, Maurilio; Zamò, Alberto; Iqbal, Javeed; Piccaluga, Pier Paolo; Neri, Antonino; Chan, Wing C; Pileri, Stefano; Bertoni, Francesco; Inghirami, Giorgio; Piva, Roberto.
Blood; 120(6): 1274-81, 2012 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-22740451
Anaplastic large-cell lymphomas (ALCLs) are a group of clinically and biologically heterogeneous diseases including the ALK(+) and ALK(-) systemic forms. Whereas ALK(+) ALCLs are molecularly characterized and can be readily diagnosed, specific immunophenotypic or genetic features to define ALK(-) ALCL are missing, and their distinction from other T-cell non-Hodgkin lymphomas (T-NHLs) remains controversial. In the present study, we undertook a transcriptional profiling meta-analysis of 309 cases, including ALCL and other primary T-NHL samples. Pathway discovery and prediction analyses defined a minimum set of genes capable of recognizing ALK(-) ALCL. Application of quantitative RT-PCR in independent datasets from cryopreserved and formalin-fixed paraffin-embedded samples validated a 3-gene model (TNFRSF8, BATF3, and TMOD1) able to successfully separate ALK(-) ALCL from peripheral T-cell lymphoma not otherwise specified, with overall accuracy near 97%. In conclusion, our data justify the possibility of translating quantitative RT-PCR protocols to routine clinical settings as a new approach to objectively dissect T-NHL and to select more appropriate therapeutic protocols.
Selo DaSilva