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Performance of HIV-1 DNA or HIV-1 RNA tests for early diagnosis of perinatal HIV-1 infection during anti-retroviral prophylaxis.

Burgard, Marianne; Blanche, Stéphane; Jasseron, Carine; Descamps, Philippe; Allemon, Marie-Christine; Ciraru-Vigneron, Nicole; Floch, Corinne; Heller-Roussin, Brigitte; Lachassinne, Eric; Mazy, Fabienne; Warszawski, Josiane; Rouzioux, Christine.
J Pediatr; 160(1): 60-6.e1, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21868029

OBJECTIVE:

To compare performance of testing for human immunodeficiency virus (HIV)-1 DNA and HIV-1 RNA for diagnosis of HIV-1 infection in infants receiving preventive antiretroviral therapy.

STUDY DESIGN:

This substudy of the French multicenter prospective cohort of neonates born to HIV-infected mothers, included 1567 infants tested for HIV with polymerase chain reaction (PCR) in a single laboratory, receiving post-natal prophylaxis, not breastfed, and having simultaneous HIV-1 DNA and RNA results before 45 days. The performance of PCR was assessed in reference to the 6-month HIV-1 RNA result.

RESULTS:

Specificity of both HIV-1 RNA and HIV-1 DNA PCR was 100% at all ages (except 99.8% for DNA at birth); sensitivity was 58% (RNA) and 55% (DNA) at birth, and 89% at 1 month, 100% at 3 months for both, and 100% at 6 months (DNA). Concordance between HIV-1 DNA and RNA results was 0.78 and 0.81 (Kappa) at birth and 1 month and 100% at 3 and 6 months. Type of maternal and neonatal prophylaxis had no effect on sensitivity, but influenced viral load.

CONCLUSION:

The performances of testing for HIV-1 DNA and RNA were similar with 100% sensitivity at 3 months. At 1 month during prophylaxis, 11% of infected children had negative PCR results.
Selo DaSilva