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Carrier-mediated uptake of Levofloxacin by BeWo cells, a human trophoblast cell line.

Polachek, Hana; Holcberg, Gershon; Polachek, Joseph; Rubin, Mazal; Feinshtein, Valeria; Sheiner, Eyal; Ben-Zvi, Zvi.
Arch Gynecol Obstet; 281(5): 833-8, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-19629508

OBJECTIVE:

Placental transfer of Levofloxacin (LF), a broad spectrum fluoroquinolone antibiotic, and its inhibition was investigated in BeWo cells, a human trophoblast cell line.

METHODS:

The experiments of LF uptake by BeWo cells were performed after preincubation and in the presence of the P-glycoprotein inhibitors (Cyclosporin A, Verapamil and Quercetin), the organic anion/cation transporter inhibitor (Cimetidine) and the MCT substrates (lactic acid and salicylic acid).

RESULTS:

P-glycoprotein inhibitors increased the uptake of LF by BeWo cells. The increase in LF accumulation by Cyclosporin A, Verapamil and Quercetin was by 30, 90 and 80%, respectively. Cimetidine, the organic cation inhibitor, increased the transport of LF by 48%. Lactic acid and salicylic acid, the MCT substrates, initially decreased the accumulation of LF by 30% and subsequently increased the uptake of LF by 500 and 53%, respectively.

CONCLUSIONS:

The uptake of LF by human trophoblast cells is mediated by multiple transporters as well as passive diffusion.
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