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Toll-like receptors 4 contribute to endothelial injury and inflammation in hemorrhagic shock in mice.

Benhamou, Ygal; Favre, Julie; Musette, Philippe; Renet, Sylvanie; Thuillez, Christian; Richard, Vincent; Tamion, Fabienne.
Crit Care Med; 37(5): 1724-8, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19325486

OBJECTIVE:

Hemorrhagic shock followed by resuscitation (HS/R) promotes organ injury by priming cells of the innate immune system for inflammatory response. Toll-like receptors (TLRs) play an important role in signal transduction in shock/resuscitation conditions. Because proinflammatory mediators are a critical event in mesenteric endothelial injury induced by HS/R, we assessed the role of TLR4 or TLR2 in this setting.

DESIGN:

Laboratory investigation.SETTING: Research laboratory at Rouen University Medical School.

SUBJECTS:

Male wild-type, TLR4(-/-) and TLR2(-/-) mice with the same C57BL/6 background.INTERVENTIONS: Mice were submitted to 30 minutes hemorrhagic shock followed by 1 hour resuscitation, after which mesenteric endothelial dysfunction, microvascular injury, and TNF[alpha] production were assessed.

MEASUREMENTS AND MAIN RESULTS:

HS/R markedly decreased nitric oxide-mediated mesenteric relaxations induced by acetylcholine, assessed ex vivo on a myograph. By contrast, in TLR4-deficient mice, HS/R did not impair the nitric oxide-mediated responses to acetylcholine. No protection was observed in TLR2-deficient mice. TLR4-deficient mice also displayed a significant reduction in fluid resuscitation and TNF[alpha] systemic production.

CONCLUSIONS:

TLR4 contributes to mesenteric endothelial dysfunction after hemorrhagic shock. This early TLR4-induced vascular injury may be an important trigger of the systemic inflammatory response occurring in this disease.
Selo DaSilva