Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions.
Kuipers, Grietje; Peschke, Markus; Ismail, Nurzian Bernsel; Hjelm, Anna; Schlegel, Susan; Vikström, David; Luirink, Joen; de Gier, Jan-Willem.
Methods Mol Biol
; 1586: 109-126, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28470601
Rapid assembly of multiple DNA fragments through direct transformation of PCR products into E. coli and Lactobacillus.
A series of new E. coli-Thermococcus shuttle vectors compatible with previously existing vectors.
A dual-site gateway cloning system for simultaneous cloning of two genes for plant transformation.
A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants.
A gain-of-function positive-selection expression plasmid that enables high-efficiency cloning.
Mitochondrial Gene Therapy: Advances in Mitochondrial Gene Cloning, Plasmid Production, and Nanosystems Targeted to Mitochondria.
Cloning and expression of rice glutamate decarboxylase (GAD) in Escherichia coli.
Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis.
Characterizing seamless ligation cloning extract for synthetic biological applications.
Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.