A discontinuous RNA platform mediates RNA virus replication: building an integrated model for RNA-based regulation of viral processes.
Wu, Baodong; Pogany, Judit; Na, Hong; Nicholson, Beth L; Nagy, Peter D; White, K Andrew.
; 5(3): e1000323, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19266082
Conserved motifs in a tombusvirus polymerase modulate genome replication, subgenomic transcription, and amplification of defective interfering RNAs.
Defining the roles of cis-acting RNA elements in tombusvirus replicase assembly in vitro.
Tombusvirus Y-shaped translational enhancer forms a complex with eIF4F and can be functionally replaced by heterologous translational enhancers.
Assembly-hub function of ER-localized SNARE proteins in biogenesis of tombusvirus replication compartment.
Recruitment of Vps34 PI3K and enrichment of PI3P phosphoinositide in the viral replication compartment is crucial for replication of a positive-strand RNA virus.
The role of co-opted ESCRT proteins and lipid factors in protection of tombusviral double-stranded RNA replication intermediate against reconstituted RNAi in yeast.
Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast.
The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating assembly of the viral replicase complex.
The TPR domain in the host Cyp40-like cyclophilin binds to the viral replication protein and inhibits the assembly of the tombusviral replicase.
Cyclophilin A binds to the viral RNA and replication proteins, resulting in inhibition of tombusviral replicase assembly.